FLUORESCENCE CELL COUNTER
LUNA-STEM™ Automated Fluorescence Cell
Counter for Stem Cells & SVF

The LUNA-STEM™ takes adipose-derived stem cell and SVF samples to count live nucleated cells, dead nucleated cells, and non-nucleated cells with precision and consistency for downstream procedures.

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When using tissue-derived cells such as stem cells or the cells from the adipose stromal vascular fraction (SVF), knowing cell concentration and viability is crucial to ensure and control the quality of downstream procedures. Tissue-derived samples are often contaminated with red blood cells and noncellular debris, making cell counting a time-consuming, subjective, and error-prone process. The LUNA-STEM™ is equipped with dual fluorescence and brightfield optics to distinguish live and dead nucleated cells from non-nucleated cells. Samples are stained with nucleic acid dyes to determine the concentration and viability of mononucleated cells. Non-nucleated cells are distinguished from debris and also counted. In as little as 30 seconds, the LUNA-STEM™ reports total cell concentration, nucleated cell concentration, non-nucleated cell concentration, cell viability, and average cell size. A high-resolution image with live cells labeled with green circles and dead cells with red circles can be viewed to confirm data visually.

Count Tissue-Derived Cells
Using dual fluorescence and brightfield optics, the LUNA-STEM™ can distinguish between cells and debris.
30 Seconds To Results
In as little as 30 seconds, the LUNA-STEM™ captures, merges, and analyzes images from three channels to produce cell count and viability data.
Nucleated And Non-Nucleated Cell Analysis
Nucleic stains make sure that nucleated cells are counted as live or dead cells whereas nonnucleated cells are counted as non-nucleated cells.
Easy Record Keeping
PDF reports complete with images and data are automatically generated and can be transferred to a PC or printed for your records.
The Lowest Cost. The Greatest Convenience.
Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA™ Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
What Do Our Customers Have To Say?
Testimonials
(5.0)
Chase Haserot

Consistent and accurate day after day!The LUNA-STEM Counter is very quick, effective, and accurate. We use the LUNA-STEM Counter on a daily basis and have run into no troubles for 2 years now. In addition we are a consumer of the LUNA-STEM Slides and AOPI Fluorescent dye, both of which are very inexpensive and extremely obtainable - with help from outstanding customer service. Definitely worth the money.

(5.0)
Albert Brell

Best cell counting. Reliable and easy to use.We use it to count mononucleated cells. Very useful and easy to use. Perfect for our daily use.

Hospital de la Santa Creu i Sant Pau

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Where The LUNA-STEM™ Has Been Cited
Citations

Methods and strategies for procurement, isolation, characterization, and assessment of senescence of human mesenchymal stem cells from adipose tissue.

2019. Gaur M, Dobke M, Lunyak VV. Methods in Molecular Biology.

Serial injections of cryopreserved fat at −196°c for tissue rejuvenation, scar treatment, and volume augmentation.

2018. Ohashi M, Chiba A, Nakai H, Fukuda E, Higuchi T. Plastic and Reconstructive Surgery-Global Open, 6(5): e1742.

Comparison of two automated cell counters for enumeration and viability of mesenchymal stem cells for clinical cellular therapy trials

2018. Radel DJ, Madde P, Dietz A. Cytotherapy, 20(5): S69–S70.

Therapeutic potential of adipose-derived therapeutic factor concentrate for treating critical limb ischemia.

2016. Procházka V, Jurčíková J, Laššák O, Vítková K, Pavliska L, Porubová L, Buszman PP, Krauze A, Fernandez C, Jalůvka F, Špačková I, Lochman I, Jana D, Merfeld-Clauss S, March KL, Traktuev DO, Johnstone BH. Cell transplantation. 25(9): 1623-1633.

The safety and efficacy of cell-assisted fat grafting to traditional fat grafting in the anterior mid-face: an indirect assessment by 3D imaging.

2015. Sasaki GH. Aesthetic Plastic Surgery, 39(6):833-46.

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Resources
Downloads
Logos Biosystems Products
brochure

2022-04-07 | 2.14 MB

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LUNA-STEM™
brochure

2022-04-07 | 704 KB

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LUNA-STEM™
Quick Start Guide

2021-06-18 | 249.67 KB

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Using the LUNA Reusable Slide for accurate cell counting with automated cell counters
Application note

2018-04-13 | 665.84 KB

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How to Count Cells An Overview of Cell Counting Methods
Application note

2018-04-13 | 720.79 KB

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Ordering Information
Cat #
Product
Qty
L30001
LUNA-STEM™ Automated Fluorescence Cell Counter for Stem Cells & SVF
1 unit
L12005
PhotonSlide™, 50 Slides
1 box
L12006
PhotonSlide™, 500 Slides
10 boxes
L12007
PhotonSlide™, 1000 Slides
20 boxes
L12011
LUNA™ Reusable Slide
1 unit
L12012
LUNA™ Reusable Slides
2 units
L12014
LUNA™ Reusable Slide Coverslips
10 units
F23001
Acridine Orange/Propidium Iodide Stain
2 x 0.5 mL
F23002
Acridine Orange Stain
2 x 0.5 mL
F23003
Propidium Iodide Stain
2 x 0.5 mL
F23212
Cell Dilution Buffer
5 x 20 mL
F23102
LUNA™ Fluorescence Calibration Beads
1 x 0.5 mL
L34003
LUNA-STEM™ IQ/OQ Protocol
1 copy
P10001
LUNA™ Printer I
1 unit
P12001
LUNA™ Printer I Paper (Thermal, 275 prints/roll, 6rolls)
3 x 2 rolls
Specifications
test
test