With a growing emphasis on both inter- and intra-lab reproducibility in biomedical research, the seemingly simple act of obtaining accurate cell counts is now more important than ever. In addition to negatively affecting reproducibility, inaccurate cell counts have downstream consequences for determining the potency and/or efficacy of cell therapy treatments, disease diagnoses, rate of growth for regenerated tissues, and various bioassays [1]. Traditionally, the gold standard for ascertaining viable cell numbers has involved placing cells stained with a vital dye such as trypan blue in a hemocytometer and manually counting them under a microscope [2-5]. Manually counting cells has a low cost per count and provides versatility [2], but the procedure is time consuming and labor intensive. Additionally, the results obtained from manual counts may suffer from cross-contamination through the reusable hemocytometer and significant variations may arise from different hemocytometer volumes, pipetting inconsistencies, and inter-user variation [2,3,5]. Recent advances in automated cell counting systems such as the LUNA-II™ from Logos Biosystems make it possible to obtain more consistent, accurate, and reproducible results at a reasonable cost per count [2-4].
The LUNA-II™ Automated Cell Counter is an advanced cell counting system with a state-of-the-art liquid lens technology that allows for autofocusing. The LUNA-II™ rapidly performs highly accurate cell counts by employing statistical observations of the intensity characteristics in live and dead cells. Liquid lenses have several advantages over other traditional optical lenses: reduced size, large range of optical variation, long lifetime, shock resistance, speed, and low power consumption [6,7]. Due to the liquid lens and advanced counting algorithm, the LUNA-II™ also provides considerably lower levels of variability in cell concentration and viability determinations. Technical variability in cell counting may occur when repeated measurements of the same sample are 1) made with a single device (intra) or 2) made with different devices (inter). The advanced engineering and high quality control exhibited in the LUNA-II™ results in a low level of device-to-device variability, ensuring both intra- and inter-lab consistency and reproducibility. Here we demonstrate how the LUNA-II™ will help you obtain more accurate and consistent cell counts in various experimental settings.
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2018-04-13
2018-04-13
2018-12-05
2018-12-05
2018-12-05
